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PCR & RT PCR
Polymerase chain reaction (PCR) is an important tool for the detection od DNA and RNA with application across all biotechnology / molecular biology research, including forensics, gene expression, identification and discovery. Tradional PCR has relied on agorose gelbased end point detection of the reaction, in the plateau phase, This has been time consuming, not very precise and with a degree of sample to samples variability. Real time PCR combines the amplification and detection steps into one single procedure, saving time. Real time PCR measures sample during the exponential phase of the PCR reaction and produces results while the reaction is in progess. This achives detection accuracy from very low initial concentrations of starting material. Real Time PCR is sufficiently sensitive to detect as low as 2-fold changes, allowing discrimination in yields that are not possible to achieve with Agarose gels. |